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Recombinant Cynomolgus CD7 Protein

來(lái)源: 發(fā)布時(shí)間:2024-04-11

重組腸激酶(rEK)是一種高純度的重組牛腸激酶輕鏈片段,氨基酸序列與牛腸激酶輕鏈一致,有著和天然提取的腸激酶同樣特異的酶切位點(diǎn),切割位點(diǎn)Asp-Asp-Asp-Asp-Lys,可去除位于蛋白N-末端的融合蛋白,以除去不需要的融合標(biāo)簽,同時(shí)重組腸激酶(rEK)具有比天然酶更高的切割活性。重組腸激酶為采用重組大腸桿菌分泌表達(dá)的高純度、高活性、高特異的牛腸激酶,不含其他蛋白酶,可以在較寬pH范圍(4.5-9.5)和較寬溫度范圍內(nèi)有效切割融合蛋白,并且在各種去垢劑和變性劑存在的條件下仍具有部分活性。本品不含標(biāo)簽,由于具有極高酶切活性,酶切反應(yīng)使用量少,不影響下游蛋白應(yīng)用,可不考慮除去。產(chǎn)品信息規(guī)格100U/200U/500U/1000U/5000U產(chǎn)品性質(zhì)來(lái)源(Source)大腸桿菌表達(dá)分子量(MolecularWeight)理論值25.85kDa外觀(Appearance)澄清、無(wú)色至淡黃色液體酶濃度(EnzymeConcentration)≥5U/uL活性定義(ActivityDefinition)一個(gè)活性單位定義為25°C,12-16h,通過(guò)研究,CDNF可防止6-羥基多巴胺(6-OHDA)誘導(dǎo)的多巴胺能神經(jīng)元變性,這可能對(duì)帕金森氏病有益。Recombinant Cynomolgus CD7 Protein,His Tag

Recombinant Cynomolgus CD7 Protein,His Tag,標(biāo)準(zhǔn)物質(zhì)

CD40,alsoknownasTNFRSF5,isa45-50kDatypeItransmembraneglycoproteinmemberoftheTNFreceptorsuperfamily.MaturehumanCD40consistsofa173aminoacid(aa)extracellulardomain,atransmembranedomain,anda62aacytoplasmicdomain.CD40servesmultiplefunctionsinbothhematopoieticandepithelialcancersandisatargetfortumorimmunotherapy.DysregulationofCD40/CD40LigandexpressionandinteractionscontributestotheimmunedeficiencyassociatedwithHIVinfectionandAIDS.Itisalsoimplicatedinthepathologyofmultiplecardiovasculardiseasesincludingatherosclerosis,atherothrombosis,andrestenosis.產(chǎn)品性質(zhì)別名CD40;CD40Lreceptor;TNFRSF5;CD40antigen;CD40molecule;CDw40;MGC9013;UniprotNo.P25942表達(dá)區(qū)間及表達(dá)系統(tǒng)RecombinantBiotinylatedHumanCD40/TNFRSF5ProteinisexpressedfromHEK293CellswithHistagandAvitagattheC-terminal.ItcontainsGlu21-Arg193.分子量Approximately22.1kDa.Duetoglycosylation,theproteinmigratesto35-40kDabasedonTris-BisPAGEresult.純度>95%asdeterminedbySDS-PAGEandHPLC.活性Recombinant Human Transferrin R/CD71 Protein,His-Avi Tag在分子生物學(xué)、生物化學(xué)或生物工程制藥研究中也常作為工具酶用于重組融合蛋白的特異性斷裂。

Recombinant Cynomolgus CD7 Protein,His Tag,標(biāo)準(zhǔn)物質(zhì)

Recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein (Primary Amine Labeling),hFc Tag性能參數(shù)分子別名(Synonyms)OX40Lreceptor;CD134;TNFRSF4;Ly-70表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling)isexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsLeu29-Ala216.[Accession|P43489]分子量大小(MolecularWeight)TheproteinhasapredictedMWof46.8kDa.Duetoglycosylation,theproteinmigratesto72-75kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGE制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).儲(chǔ)存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.

透明質(zhì)酸酶,英文名稱Hyaluronidase,一類能夠降低體內(nèi)透明質(zhì)酸活性的酶的總稱,結(jié)構(gòu)上由4個(gè)相同亞基構(gòu)成,每個(gè)亞基分子量接近14kDa,該酶是一種糖蛋白,包含5%的甘露糖和2.2%的葡萄糖胺。功能上該酶可隨機(jī)裂解透明質(zhì)酸、軟骨素和硫酸軟骨素中的β-N-乙酰己糖胺-[1→4]糖苷鍵。常與膠原酶聯(lián)合使用于降解細(xì)胞外基質(zhì),從而從組織中分離活細(xì)胞。本品來(lái)源于牛睪丸,活力值為400–1,000units/mg,合適的pH值為4.5–6.0。產(chǎn)品性質(zhì)中文別名(ChineseSynonym)玻璃糖醛酸酶;玻璃酸酶英文別名(EnglishSynonym)Hyaluronoglucosaminidase;Hyaluronate4-glycanohydrolaseCAS號(hào)(CASNO.)37326-33-3來(lái)源(Source)牛睪丸分子量(MolecularWeight)~55kDa類型(Type)I-S外觀(Appearance)淡黃色至米黃色至褐色粉末糖苷酶 F (PNGase F)是一種酰胺水解酶,經(jīng)過(guò)和平空間站伊麗莎菌克隆,主要由腦膜炎膿桿菌等革蘭氏陰性菌分泌。

Recombinant Cynomolgus CD7 Protein,His Tag,標(biāo)準(zhǔn)物質(zhì)

Coronavirusesarecommonlycomprisedoffourstructuralproteins:Spikeprotein(S),Envelopeprotein(E),Membraneprotein(M)andNucleocapsidprotein(N).TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheSproteinishomotrimeric,witheach~180-kDamonomerconsistingoftwosubunits,S1andS2.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).產(chǎn)品性質(zhì)別名SproteinRBD;SglycoproteinRBD;SpikeproteinRBDAccessionQHD43416.1表達(dá)區(qū)間及表達(dá)系統(tǒng)RecombinantSARS-CoV-2SpikeRBD(OmicronBA.4/BA.5/BA.5.1.3/BA.5.2)ProteinisexpressedfromExpi293withHistagattheC-terminal.ItcontainsArg319-Phe541(G339D,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,L452R,S477N,T478K,E484A,F486V,Q498R,N501Y,Y505H).rTEV是一種用來(lái)切除融合蛋白上親和標(biāo)簽的常用工具酶,具有很強(qiáng)的位點(diǎn)特異性,嚴(yán)格識(shí)別七氨基酸序列。Xenopsin

IdeS 具有獨(dú)特的底物選擇性,可以作為工具酶應(yīng)用于抗體類藥物或抗體融合蛋白藥物的結(jié)構(gòu)表征分析。Recombinant Cynomolgus CD7 Protein,His Tag

Recombinant Biotinylated Human KIR2DL1 Protein,His-Avi Tag性能參數(shù)分子別名(Synonyms)CD158A;CD158Ankat1;cl-42表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲(chǔ)存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.Recombinant Cynomolgus CD7 Protein,His Tag

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